Molecular phylogeny of brachiopods and phoronids based on nuclear-encoded small subunit ribosomal RNA gene sequences
نویسندگان
چکیده
Molecular phylogeny of brachiopods and phoronids based on nuclear-encoded small subunit ribosomal RNA gene sequences. CONTENTS PAGE 1. Introduction 2040 2. Materials and methods 2043 (a) Specimens 2043 (b) Isolation of DNA 2043 (c) Polymerase chain reaction ampli¢cation, puri¢cation and sequencing of SSU rRNA 2043 (d) Sequence alignment and masking 2044 (e) Parametric outgroup selection 2044 (f) Phylogenetic analyses 2044 (g) Estimation of evolutionary distances and rates 2044 3. Results 2045 (a) Sequence reliability and alignment parameters 2045 (b) Phylogenetic reconstructions 2045 (c) Correlation of genetic distance with classi¢cation 2050 (d) Exclusion of the sequence from Phoronis vancouverensis 2050 (e) Rate and time-course of molecular evolution 2052 4. Discussion 2054 (a) Molecular phylogenetic analyses 2054 (b) Correlation of molecular and morphological phylogenies 2055 (c) Evolutionary and biogeographic inferences 2057 5. Conclusions 2058 References 2059
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